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American Journal of Innovative Research & Applied Sciences
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  | ARTICLES | Am. J. innov. res. appl. sci. Volume 9,  Issue 1, Pages 30-32 (July 2019)
Research Article
 
RESUME


Introduction :
Les plantes sont largement utilisées par les populations africaines pour se soigner.
L’étude de la toxicité des extraits de plante s’avère nécessaire. Objectif : l’objectif de cette étude est de donner ne méthode in vitro permettant d’évaluer la toxicité des extraits de plante. Méthode : La méthode utilisée est celle de la culture de cellules souches en présence de MTT(bromure de 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium). Conclusion : Le test du MTT a permis d’évaluer in vitro la toxicité des extraits de plantes avant leur utilisation sur des animaux de laboratoire.
Mots-clés: Cellules souches, MTT, Toxicité.


ABSTRACT


Introduction: Plants are widely used by African populations for healing. The study of the toxicity of plant extracts is necessary. Objective: The objective of this study is to provide an in vitro method to evaluate the toxicity of plant extracts. Method: The method used is that of cell culture in the presence of TTM (bromure de 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium). Conclusion: The MTT test assessed in vitro the toxicity of plant extracts before their use on laboratory animals.
Keywords: Stem cells, MTT, Toxicity.
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American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
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Authors Contact

*Correspondant author and authors Copyright © 2019:

Gueyraud Rolland Kipré 1* | Hriday Shankar Pandey 2 | Pankaj Seth | and | Allico Joseph Djaman 1,3  |


Affiliation.


1. Université Félix Houphouët-Boigny | UFR Biosciences | Laboratoire de Pharmacodynamie Biochimique | Abidjan | Côte d’Ivoire
2. National Brain Research Centre |
NH-8, Manesar, Gurugram | Inde |
3. Institut Pasteur de Côte d’Ivoire | Laboratoire de Biochimie | Abidjan | Côte d’Ivoire |


This article is made freely available as part of this journal's Open Access: ID | Kipré-Ref.6-ajira080619 |
| JULY | VOLUME 9 | N° 1 | 2019 |
TECHNIQUE D’ETUDE DE LA TOXICITE DES EXTRAITS DE PLANTE PAR LA METHODE DU MTT

TECHNIQUE FOR STUDYING THE TOXICITY OF PLANT EXTRACTS BY THE MTT METHOD

Gueyraud Rolland Kipré 1* | Hriday Shankar Pandey 2 | Pankaj Seth | and | Allico Joseph Djaman 1,3  |. Am. J. innov. res. appl. sci.  2019; 9(1):30-32.

   | PDF FULL TEXT |  | XML FILE |  | Received | 08 May 2019 | | Accepted | 22 June 2019 |  | Published | 05 July 2019 |