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  | ARTICLES | Am. J. innov. res. appl. sci. Volume 10,  Issue 1, Pages 15-23 (Januray 2020)
Research Article
 
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American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
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| JANUARY | VOLUME 10 | N° 1 | 2020 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference  CIF/15/0289M |
  American Journal of Innovative Research & Applied Sciences
Authors Contact

*Correspondant author and authors Copyright © 2020:

Nergis Kaya *1 | and | Cüneyt Aki 2 |.


Affiliation.

1. Canakkale Onsekiz Mart University | Department of Food Processing | Food Technology Program | 17200, Canakkale | Turkey |
2. Canakkale Onsekiz Mart University
| Faculty of Science and Arts | Department of Biology | Subdivision of Molecular Biology, 17020, Canakkale |Turkey

This article is made freely available as part of this journal's Open Access: ID | Nergis-Ref.1-ajiras060120   |
ABSTRACT

Background: Calendula officinalis (pot marigold) L., known for its ornamental plant characteristics, is a medicinal plant which belongs to the Asteraceae (Compositae) family. Methods: The cell suspension cultures of Calendula officinalis and C. arvensis species with pharmacological importance were created in the four different MS media which were supplemented with different concentrations of NAA:BAP (1:1, 0.5:5 mg/l) and IAA:BAP (1:1, 0.5:5 mg/l) under sterile conditions. Caffeic acid and beta carotene quantity were researched at the end of every 30 days in the cell suspension cultures (stationary phase) during a total of 120 days. Statistical analysis was performed with Tukey Multiple Comparison Test. Maximum caffeic acid and beta carotene quantity were obtained from the cell suspension culture of C. officinalis species developing in MS1 nutrient medium (268,59 µg/g dry weight). Results: It was found that all nutrient media for the cell suspension culture (MS1, MS3, MS4, MS6) of C. officinalis species had more caffeic acid and beta carotene quantity than C. officinalis species. Caffeic acid and beta carotene quantity accumulating in the cell suspension culture of C. officinalis and C. arvensis species was more than is found in the leaves of Calendula species in the literature.

Keywords: Cell suspension culture, caffeic acid, beta carotene, Calendula officinalis, Calendula arvensis.
*Corresponding author Author & Copyright Author © 2020: | Nergis Kaya |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied
Sciences are the property of Atlantic Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/
THE EFFECT OF PLANT GROWTH REGULATORS ON CAFFEIC ACID AND BETA CAROTENE QUANTITY IN THE CELL SUSPENSION CULTURES OF CALENDULA OFFICINALIS L. AND CALENDULA ARVENSIS L.

Nergis Kaya *1 | and | Cüneyt Aki 2 |. Am. J. innov. res. appl. sci. 2020; 10(1):15-23.

    | PDF FULL TEXT |  | XML FILE  | | Received | December 29, 2019 | | Accepted | January 09, 2020 |  | Published | January 15, 2020 |