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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 9, Issue 3, Pages 284-292 (September 2019) |
| Research Article |
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American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
| HOME || ABOUT US || ARCHIVES || AIMS AND SCOP || AUTHORS || REVIEW|| SUBMIT MANUSCRIPT || EDITORIAL BOARD || CONTACT US |
| SEPTEMBER | VOLUME 9 | N° 3 | 2019 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference CIF/15/0289M |
American Journal of Innovative Research & Applied Sciences
Authors Contact
*Correspondant author and authors Copyright © 2019:
| Jacques Ouédraogo *1 | Mariam Kiébré 1 | Zakaria Kiébré 1 | Boureima Sawadogo 2 | and | Pauline Bationo Kando 1 |
Affiliation.
1. Université Joseph KI-ZERBO | Equipe de Génétique et Amélioration des Espèces | Ouagadougou | Burkina Faso |
2. Université de Fada N’Gourma | Institut Supérieur des Sciences du Développement Durable | Fada N’Gourma | Burkina Faso |
This article is made freely available as part of this journal's Open Access: ID | Jacques-Ref.1-ajira170919 |
*Correspondant author and authors Copyright © 2019:
| Jacques Ouédraogo *1 | Mariam Kiébré 1 | Zakaria Kiébré 1 | Boureima Sawadogo 2 | and | Pauline Bationo Kando 1 |
Affiliation.
1. Université Joseph KI-ZERBO | Equipe de Génétique et Amélioration des Espèces | Ouagadougou | Burkina Faso |
2. Université de Fada N’Gourma | Institut Supérieur des Sciences du Développement Durable | Fada N’Gourma | Burkina Faso |
This article is made freely available as part of this journal's Open Access: ID | Jacques-Ref.1-ajira170919 |
ABSTRACT
Background: The assessment of genetic diversity is an asset for the development of any varietal conservation and improvement strategy. Methods: This work aims to establish the level and structure of genetic diversity of 54 accessions of amaranth collected in two climatic zones (Sudanese zone and Sudano-Sahelian zone) of Burkina Faso. Fourteen (14) ISSRs markers developed by eurofinmwg/ Operon were used in this study. Results: Eight (8) of them were been polymorph and used for data analysis. All eight primers showed 100% polymorphism with an average PIC of 64%. A total of 40 alleles were revealed with an average of 5 alleles by locus. Also, an average of effective allele number of 3.94, a heterozygoty expected of 0.37 and Shannon diversity indexes of 1.3 were recorded. These parameters calculated showed that the markers used are informative and revealed an important genetic variability structured in four groups (A, B, C and D) within the collection amaranth cultivated in Burkina Faso. The influence of "climatic zone" and "morphotype" factors on the level and the organization of genetic diversity is very low. Conclusion: 98.80% of total genetic diversity is due to variability intra-population.
Keywords: Amaranth, neglected species, molecular variability, assessment .
Background: The assessment of genetic diversity is an asset for the development of any varietal conservation and improvement strategy. Methods: This work aims to establish the level and structure of genetic diversity of 54 accessions of amaranth collected in two climatic zones (Sudanese zone and Sudano-Sahelian zone) of Burkina Faso. Fourteen (14) ISSRs markers developed by eurofinmwg/ Operon were used in this study. Results: Eight (8) of them were been polymorph and used for data analysis. All eight primers showed 100% polymorphism with an average PIC of 64%. A total of 40 alleles were revealed with an average of 5 alleles by locus. Also, an average of effective allele number of 3.94, a heterozygoty expected of 0.37 and Shannon diversity indexes of 1.3 were recorded. These parameters calculated showed that the markers used are informative and revealed an important genetic variability structured in four groups (A, B, C and D) within the collection amaranth cultivated in Burkina Faso. The influence of "climatic zone" and "morphotype" factors on the level and the organization of genetic diversity is very low. Conclusion: 98.80% of total genetic diversity is due to variability intra-population.
Keywords: Amaranth, neglected species, molecular variability, assessment .
*Corresponding author Author & Copyright Author © 2019: | Jacques Ouédraogo |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied Sciences are the property of Atlantic Center Research
Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/.
GENETIC DIVERSITY OF A COLLECTION OF AMARANTH (Amaranthus spp) OF BURKINA FASO USING ISSR MARKERS
| Jacques Ouédraogo *1 | Mariam Kiébré 1 | Zakaria Kiébré 1 | Boureima Sawadogo 2 | and | Pauline Bationo Kando 1 |. Am. J. innov. res. appl. sci. 2019; 9(3):284-292.
| PDF FULL TEXT | | XML FILE | | Received | 21 September 2019 | | Accepted | 26 Sepytember 2019 | | Published | 28 September 2019 |
| Jacques Ouédraogo *1 | Mariam Kiébré 1 | Zakaria Kiébré 1 | Boureima Sawadogo 2 | and | Pauline Bationo Kando 1 |. Am. J. innov. res. appl. sci. 2019; 9(3):284-292.
| PDF FULL TEXT | | XML FILE | | Received | 21 September 2019 | | Accepted | 26 Sepytember 2019 | | Published | 28 September 2019 |