This article is made freely available as part of this journal's Open Access: ID |   Hala-ManuscriptRef.1-ajiras080717 Saudia 1 |

Affiliation.

1. Ain-Shams University | Faculty of Science | Microbiology Department | Cairo | Egypt |  
2. Ahmed Maher teaching hospital | Medical Laboratory | Cairo | Egypt |
3. Jazan University | Faculty of Medicine | Medical Biology Department | Jazan | Saudia Arabia |

Authors Contact

*Correspondant author and authors Copyright © 2017:

|  Hala M. Abushady 1 | Einas H. El-Shatoury 1 | and | Al-Shimaa S. Abd-elmegeed *2,3 |
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American Journal of Innovative Research & Applied Sciences
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  | ARTICLES | Am. J. innov. res. appl. sci. Volume 5,  Issue 2, Pages 126-137 (August 2017)
Research Article
 
American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
|
| AUGUST | VOLUME 5 | N° 2 | 2017 |
ABSTRACT


Background
, medicinal plants have been used as a source of therapies since ancient times in Egypt. Objectives, the present study was designed to investigate the antibacterial, antioxidant and antitumor activity of aqueous, ethanol and acetone extracts from Curcuma longa and Origanum marjorana cultivated in Egypt. The study was also extended to identify the phenolic compounds present using HPLC then evaluate their antibacterial and antitumor activity of the most abundant compounds. Methods, the disk diffusion method and micro-broth dilution were used to determine MIC and MBC of the plant extracts against ten bacterial strains belonging to five species, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes. Phytochemical screening followed by 2,2-diphenyl-1-picrylhydrazyl assay were used to assess the antioxidant of the extracts then the large intestinal carcinoma (CACO) cell line was used to evaluate antitumor of these extracts by Sulfo-Rhodamine B colorimetric (SRB) assay
. Results, the results indicated that studied crude extracts were able to inhibit the growth of at least 5 of the tested bacteria. The two studied plants have various bioactive components and showed moderate antioxidant activity, moreover the results show that IC50 values below 20 μg/mL were recorded for the crude extract of both tested plants. Rutin, benzoic acid and salicylic acid were the most abundant phenolic compounds in C. longa and O. marjorana. Rutin and benzoic acid showed anti-bacterial activity against 100% and 80% of tested bacteria, respectively and also expressed moderate cytotoxic activity with IC50 22.7 and 47.8 µg/mL, respectively. Conclusions, the present investigation provided supportive data for the possible use of the plant extracts investigated here in treatment of various diseases.
Keywords: Antibacterial, Antioxidant, Cytotoxicity, HPLC, Medicinal plants, Phyto-chemicals.
BIOLOGICAL ACTIVITY OF CURCUMA LONGA AND ORIGANUM MARJORANA, CULTIVATED IN EGYPT

   |  Hala M. Abushady  | Einas H. El-Shatoury  | and | Al-Shimaa S. Abd-elmegeed |. Am. J. innov. res. appl. sci.  2017; 5(2):126-137.

    | PDF FULL TEXT |           | Received | 08 July 2017|          |Accepted | 20 July 2017|         |Published 24 July 2017|