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American Journal of Innovative Research & Applied Sciences
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  | ARTICLES | Am. J. innov. res. appl. sci. Volume 6,  Issue 1, Pages 29-34 (January 2018)
Research Article
 
American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
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| JANUARY | VOLUME 6 | N° 1  | 2018 |
Authors Contact

*Correspondant author and authors Copyright © 2017:

   | Nurcihan Hacıoğlu Doğru * | Çiğdem Gül  | and | Murat Tosunoğlu  |

Affiliation.


Çanakkale Onsekiz Mart University | Faculty of Arts and Sciences | Department of Biology | Canakkale | Turkey |
This article is made freely available as part of this journal's Open Access: ID | Nurcihan-ManuscriptRef.1-ajira030118 |
ABSTRACT

Background:Aquatic ecosystems contain a variety of microorganisms, of which, a limited number are able to develop in association with higher organisms and of these, a small number are pathogenic bacteria. The ability of pathogenic bacteria to cause disease in a susceptible host is determined by multiple virulence factors acting individually or together at different stages of infection. Objectives: The main goal of the present paper was to perform microbiological investigations to phenotypically emphasize the some virulence factors (enzymatic activity) of Gram negative bacteria isolated from Biga stream and some amphibian (Pelophylax ridibundus) and reptilians (Mauremys rivulata, Natrix natrix)  captured around this freshwater on April-May 2014. Methods: Their cloaca and oral samples were taken with sterile swabs, and then the animals were released to the study area. To isolate water bacterial isolates, a freshwater sample was also taken from the study area where the animal species were collected. Results:A total of 431 Gram negative bacteria were successfully isolated from cloaca and oral samples of the aquatic amphibians and reptiles as well as from the water sample. The most frequent isolate was Aeromonas hydrophila (31.09%). The total numbers of bacteria obtained were as follows: 57 in N. natrix, 51 in M. rivulata, and 50 in P. ridibundus, and 273 in the water sample. The presence of enzymes that determined virulence factors for bacterial strains was identified: DNase, amylase, lipase, haemolysin and protease. Conclusions: With reference to, A. hydrophila is thought to carry a high virulence factor due to its high DNase, amylase, lipase and protease activity.
Keywords: Virulence factor, Biga Stream, Pelophylax ridibundus, Mauremys rivulata, Natrix natrix.
SOME VIRULENCE FACTORS OF GRAM NEGATIVE BACTERIA ISOLATED FROM SOME AMPHIBIAN AND REPTILIAN SPECIES OF THE BIGA STREAM IN TURKEY

       | Nurcihan Hacıoğlu Doğru * | Çiğdem Gül | and | Murat Tosunoğlu |.  Am. J. innov. res. appl. sci. 2018; 6(1):29-34.

    | PDF FULL TEXT |             | Received |  03 January  2018 |          | Accepted | 24 January 2018 |            | Published  27 January 2018 |