| ARTICLES | Am. J. Inn. Res. Appl Sci. Volume 1, Issue 10, Pages 384-388 (December 2015)
Research Article
  | Harisu Yunusa Umar | and | Ahmad Isah Muhammad |. American Journal of Innovative Research and Applied Sciences. 2015; 1(10):384-388.

                                              |Received | 29 October 2015|          |Accepted | 01 December 2015|         |Published | 22 December 2015|
ABSTRACTS

Background: Medicinal plants are one of the major sources used in treatment of bacterial diseases Ojective: The studies aims to evaluate the antibacterial activity of Leaves
extract of Anogeissus leiocarpus and Vitex doniana on clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Methods: The Leaves of
Anogeissus leiocarpus and Vitex doniana were dried at room temperature for two (2) weeks within which uniformity was attained. The dried leaves of each of the plants
were grinded into powder separately and extracted with 95% methanol by percolation method in which the suspension was filtered using whatmann’s filter paper. Soxhlet
Extraction was also done in which fresh solvent was added and filtered continuosly to ensure that the herbal materials are fully extracted. 150mg of each plant extract were
dissolved in 10ml of the dimethyl sulphoxide (DMSO) and Stock solutions was formed, five different concentration of each of the two plants extract were made i.e
30,60,90,120 and 150mg/ml. The Antibacterial activities of these two different plants were determined by Disc diffusion method in which the prepared discs was dipped in
the extracts prepared and dried at 45oC for 1hr and then planted on the media containing test microorganisms. Results: The crude methanol extracts of the Anogeissus
leiocarpus and Vitex doniana show broad growth inhibition against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The minimum inhibition
concentration of both Anogeissus leiocaipus and Vitex doniana was found to be at 90mg/ml against S. aureus, P. aeruginosa and E.coli. Conclusion: The present
investigation shows the vital role of these extracts in the treatment of some clinical Diseases and this finding proved that traditional usage of these plants as medicine is
effective particularly to the tested organisms and this research can be used as bases for further investigations.

Keywords:: Anogeissus leiocarpus, Vitex doniana,staphylococcus aureus, pseudomonas aeruginosa and Escherichia coli.
This article is made freely available as part of this journal's Open Access: |Manuscript | Ref.1-ajiras291015 | ISSN 2429-5396|
Affiliation :

             1. Addis Ababa university | Chemical Engineering | Addis Ababa Ethiopia |
Authors Copyright © 2015: | Harisu Yunusa Umar1,2| Ahmad Isah Muhammad1|
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