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American Journal of Innovative Research & Applied Sciences
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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 4, Issue 5, Pages 195-199 (May 2017) |
American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
|
| MAY | VOLUME 4 | N° 5 | 2017 |
ABSTRACT
Backgroud: Crassula ovata (Mill.) is a medicinal plant traditionally used to treat high blood pressure in Madagascar. Objectives: This work aims to determine the pharmacological mechanism of its antihypertensive virtue, to identify and isolate the major compound and to elucidate its chemical structure. Method: The powder of dried leaves of C. ovata was extracted by maceration with 90% ethanol. The ethanol solution was depigmented by activated charcoal then filtered on Whatman’s filter paper and evaporated to dryness. This ethanol crude extract was dissolved in distilled water and then successively partitioned with dichloromethane, ethyl acetate and butanol. Two serials of column chromatography on silica gel eluted by an increasing polarity solvent system and some preparative chromatography were used to isolate the major compound of the butanol fraction. The chemical structure of the purified compound was elucidated using some spectrometric methods UPLC–MS, UPLC–UV, 1D and 2D NMR. The vaso-relaxing activity of each extract, fraction and product was assessed on the phenylephrine pre-contacted isolated rat aorta. Result: The ethanol crude extract exhibited a vaso-relaxing activity with an EC50 247.04±5µg/ml. Among the obtained four fractions, the aqueous one showed a more potent vaso-relaxing activity but the major compound was found in the butanol fraction. It was identified as a 3,4,5-trihydroxybenzoic acid or a gallic acid. It exhibits an interesting vaso-relaxing activity with an EC50 38.8±1µg/ml. Conclusion: The vaso-relaxing effect of the C. ovata leaves crude extract certainly contributes on the antihypertensive virtue of this medicinal plant. Gallic acid constitutes the major compound of its leaf. Its vaso-relaxing activity is at about 6 times more potent than that of the crude extract.
Key words: Crassula ovata, vaso-relaxing activity, Gallic acid, NMR, Spectrometric methods
Backgroud: Crassula ovata (Mill.) is a medicinal plant traditionally used to treat high blood pressure in Madagascar. Objectives: This work aims to determine the pharmacological mechanism of its antihypertensive virtue, to identify and isolate the major compound and to elucidate its chemical structure. Method: The powder of dried leaves of C. ovata was extracted by maceration with 90% ethanol. The ethanol solution was depigmented by activated charcoal then filtered on Whatman’s filter paper and evaporated to dryness. This ethanol crude extract was dissolved in distilled water and then successively partitioned with dichloromethane, ethyl acetate and butanol. Two serials of column chromatography on silica gel eluted by an increasing polarity solvent system and some preparative chromatography were used to isolate the major compound of the butanol fraction. The chemical structure of the purified compound was elucidated using some spectrometric methods UPLC–MS, UPLC–UV, 1D and 2D NMR. The vaso-relaxing activity of each extract, fraction and product was assessed on the phenylephrine pre-contacted isolated rat aorta. Result: The ethanol crude extract exhibited a vaso-relaxing activity with an EC50 247.04±5µg/ml. Among the obtained four fractions, the aqueous one showed a more potent vaso-relaxing activity but the major compound was found in the butanol fraction. It was identified as a 3,4,5-trihydroxybenzoic acid or a gallic acid. It exhibits an interesting vaso-relaxing activity with an EC50 38.8±1µg/ml. Conclusion: The vaso-relaxing effect of the C. ovata leaves crude extract certainly contributes on the antihypertensive virtue of this medicinal plant. Gallic acid constitutes the major compound of its leaf. Its vaso-relaxing activity is at about 6 times more potent than that of the crude extract.
Key words: Crassula ovata, vaso-relaxing activity, Gallic acid, NMR, Spectrometric methods
*Correspondant author and authors Copyright © 2017:
| Nantenaina Tombozara 1 | Zoarilala Rinah Razafindrakoto 2 | David Ramanitrahasimbola 2,3 | Reine Dorothée Ramilison Razafimahefa 1 | Eric Marchioni 4 | and | Dina Andriamahavola Rakotondramanana * 3 |
| Nantenaina Tombozara 1 | Zoarilala Rinah Razafindrakoto 2 | David Ramanitrahasimbola 2,3 | Reine Dorothée Ramilison Razafimahefa 1 | Eric Marchioni 4 | and | Dina Andriamahavola Rakotondramanana * 3 |
Authors Contact
Affiliation.
1. University of Antananarivo | Faculty of Sciences, Organic Chemistry Department | Laboratory of Applied Chemistry to Natural Substances | Antananarivo | Madagascar |
2. Malagasy Institute of Applied Research | Laboratory of Applied Pharmacognosy | Itaosy | Madagascar |
3. University of Antananarivo | Faculty of Medicine, Pharmacy Department | Antananarivo | Madagascar |
4. University of Strasbourg | Laboratory of Analytical Chemistry of Bioactive Molecules | Strasbourg | France |
1. University of Antananarivo | Faculty of Sciences, Organic Chemistry Department | Laboratory of Applied Chemistry to Natural Substances | Antananarivo | Madagascar |
2. Malagasy Institute of Applied Research | Laboratory of Applied Pharmacognosy | Itaosy | Madagascar |
3. University of Antananarivo | Faculty of Medicine, Pharmacy Department | Antananarivo | Madagascar |
4. University of Strasbourg | Laboratory of Analytical Chemistry of Bioactive Molecules | Strasbourg | France |
This article is made freely available as part of this journal's Open Access: ID | Andriamahavola-ManuscriptRef.1-ajira230417 |
| ISSN: 2429-5396 (e) | www.american-jiras.com | |
| Web Site Form: v 0.1.05 | JF 22 Cours, Wellington le Clairval, Lillebonne | France |
| Web Site Form: v 0.1.05 | JF 22 Cours, Wellington le Clairval, Lillebonne | France |
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Research Article
ISOLATION OF THE GALLIC ACID IN THE BUTANOLIC FRACTION OF Crassula ovata (Mill.) DRUCE (CRASSULACEAE) LEAVES AND ITS VASO-RELAXING EFFECT
| Nantenaina Tombozara | Zoarilala Rinah Razafindrakoto | David Ramanitrahasimbola | Reine Dorothée Ramilison Razafimahefa | Eric Marchioni | and | Dina Andriamahavola Rakotondramanana |. Am. J. innov. res. appl. sci. 2017; 4(5):200-207.
| PDF FULL TEXT | |Received | 23 April 2017| |Accepted | 07 May 2017| |Published 15 May 2017 |
ISOLATION OF THE GALLIC ACID IN THE BUTANOLIC FRACTION OF Crassula ovata (Mill.) DRUCE (CRASSULACEAE) LEAVES AND ITS VASO-RELAXING EFFECT
| Nantenaina Tombozara | Zoarilala Rinah Razafindrakoto | David Ramanitrahasimbola | Reine Dorothée Ramilison Razafimahefa | Eric Marchioni | and | Dina Andriamahavola Rakotondramanana |. Am. J. innov. res. appl. sci. 2017; 4(5):200-207.
| PDF FULL TEXT | |Received | 23 April 2017| |Accepted | 07 May 2017| |Published 15 May 2017 |