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American Journal of Innovative Research & Applied Sciences
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  | ARTICLES | Am. J. innov. res. appl. sci. Volume 5,  Issue 5, Pages 370-376 (November 2017)
Research Article
 
American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
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| NOVEMBER | VOLUME 5 | N° 5  | 2017 |
Authors Contact

*Correspondant author and authors Copyright © 2017:

Rachid M’saouar | Amin Laglaoui |  Mohammed Bakkali | and | Abdelhay Arakrak * |


Affiliation.

Faculté des Sciences et Techniques | Biology department | Tangier | Morocco | Equipe de Recherche de Biotechnologies et Génie des Biomolécules (ERBGB) |

This article is made freely available as part of this journal's Open Access: ID | Arakrak-ManuscriptRef.1-ajira101017 |
ABSTRACT

Background: Hedysarum flexuosum  L; a Mediterranean herbaceous legume, participates in the soil conservation and has the ability to fix its own nitrogen, however its potential use as forage plant was emphasized. In the northwest of Morocco its populations are submitted to an increasing anthropological pressure. Objectives: The aim of this paper is to describe the status of the arbuscular mycorrhizal fungi (AMF) of Hedysarum flexuosum L; in the northwest of Morocco. A detailed description of the mycorrhizal associations in this species soil and roots is reported for the first time in this study. Materials and Methods: Soil and roots samples were extracted from two sites across the northwest of Morocco: Achakar (AC) and Khandak Lihoudi (KH). The frequency and the intensity of the (AMF) inside the roots were measured and the spores were extracted from the soil and counted. We assessed the mycorrhizal potential of the indigenous soils by using the Most Probable Number (MPN) approach. Results: We found that the number of spores was in the order of 1230/100g of soil in the site of (KH) and of 1290 in the site of (AC), for this parameter we didn’t note a significant statistical difference between the two sites. The (MPN) per 100 g of soil was 14000 in (AC) and 280 in (KH).The spores collected belong to three genera: Scutellospora, Glomus and Septoglomus. The examination of the roots showed that all of them were mycorrhized, the average frequency reach 91% in (KH), and 70% in (AC). The roots had typical mycorrhizal structures (arbuscules, hyphae, intraradical spores and vesicles). Conclusion: These results open up many opportunities for the application of the controlled mycorrhization of this forage in nurseries, the diversity of arbuscular mycorrhizal fungi present in the rhizosphere can be selected and used in improving the production of vigorous plants. It will allow its preservation and introduction in degraded areas.

KEYWORDS: Hedysarum flexuosum L, Scutellospora, Glomus, mycorrhizal rate, (AMF) infective propagules.
MYCORRHIZAL STATUS OF Hedysarum Flexuosum L; IN THE NORTHWEST OF MOROCCO

| Rachid M’saouar | Amin Laglaoui | Mohammed Bakkali | and | Abdelhay Arakrak * |. Am. J. innov. res. appl. sci. 2017; 5(5):370-376.

    | PDF FULL TEXT |              | Received | 10 October 2017|          | Accepted | 30 October 2017 |         | Published 07 November 2017 |