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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 11, Issue 3, Pages 131-136 (September, 2020) |
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American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
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| JUJY | VOLUME 11 | N° 3 | 2020 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference CIF/15/0289M |
American Journal of Innovative Research & Applied Sciences
Authors Contact
*Correspondant author and authors Copyright © 2020:
| Lahadson Stanislas Fulgence 1* | Randrianjatovo Mamy 1 | Rabemandroso Faramalala Louise 1 | Toghueo Kouipou Rufin
Marie 3 | Rakotoarison Olivier 2 | Ravelomanantsoa Germier 4 | Randrianasolo Rivoarison 1 | Nkengfack Ephrem Augustin 5 |
et | Rasoanaivo Herilala Léa 1 |
Affiliation.
1. University of Antananarivo | Faculty of Sciences | Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique
(LCSN/COB)| Antananarivo | Madagascar |
2.University of Antananarivo | Faculty of Sciences | Antananarivo | Madagascar | Laboratoire de Pharmacodynamie |
3.University of Yaoundé I | Faculty of Sciences | Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal
Plants Studies | Yaoundé | Cameroon |
4.University of Antananarivo | Faculty of Sciences | Laboratoire de Physique nucléaire et physique de l’environnement | Antananarivo |
Madagascar |
5.University of Yaoundé I | Faculty of Sciences | Laboratoire de Chimie Organique Structurale et Analytique | Yaoundé | Cameroon |
This article is made freely available as part of this journal's Open Access: ID | Lahadson-Ref1-ajira290720 |
ABSTRACT
Background: Erythroxylum ferrugineum Cav. (Erythroxylaceae) is a small tree endemic of Madagascar used in the form of decoction in traditional medicine for the treatment of dysentery. Objectives: The main aim of this study was to investigate the potential inhibitory activity of crude extracts and isolated compounds against causative agents of diarrheal Disease. Methods: Plant extracts and compounds were obtained from the air-dried powder of leaves of E. ferrugineum by cold maceration and liquid chromatographic technics. Pure compounds were identified by direct comparison of their physical and spectroscopic characteristics with those published in the literature. Effect of crude extract on intestinal motility was evaluated in vivo by intestinal transit test. Antibacterial activities were tested using micro broth dilution method. The cytotoxicity of the most active fraction and isolated compounds was assessed on vero cells lines using the MTT assay. Results: E. ferrugineum leaves extract yielded 40.05 %, slightly affected the intestinal motility and showed a moderate antibacterial activity against S. typhi, S. enterica and S. aureus; with minimum inhibitory concentration (MIC) ranging from 125 to 250 μg/ml. Its polar part (86.63 %), was non-cytotoxic (50% cytotoxic concentrations: CC50 > 200 µg/mL) and displayed the strongest antibacterial activity (MIC=100μg/mL). A phytochemical investigation of the DCM extract led to the isolation of a non-toxic new diterpene named ent-kauran-15α, 16β, 17-triol (CC50 > 200 µg/mL) and a known derivative identified ent-kauran-16β, 17-diol (CC50=67.040 ± 1.257 μg / mL), which had a MIC of 100 μg / ml against S. aureus and 2 strains of Salmonella. Conclusions: The results of this study contribute to validate the traditional use of E. ferrugineum. The chemical structures of the compounds obtained may aid the design of antibacterial agents.
Keywords: Erythroxylum ferrugineum, antibacterial, Cytotoxicity, diterpenes.
Background: Erythroxylum ferrugineum Cav. (Erythroxylaceae) is a small tree endemic of Madagascar used in the form of decoction in traditional medicine for the treatment of dysentery. Objectives: The main aim of this study was to investigate the potential inhibitory activity of crude extracts and isolated compounds against causative agents of diarrheal Disease. Methods: Plant extracts and compounds were obtained from the air-dried powder of leaves of E. ferrugineum by cold maceration and liquid chromatographic technics. Pure compounds were identified by direct comparison of their physical and spectroscopic characteristics with those published in the literature. Effect of crude extract on intestinal motility was evaluated in vivo by intestinal transit test. Antibacterial activities were tested using micro broth dilution method. The cytotoxicity of the most active fraction and isolated compounds was assessed on vero cells lines using the MTT assay. Results: E. ferrugineum leaves extract yielded 40.05 %, slightly affected the intestinal motility and showed a moderate antibacterial activity against S. typhi, S. enterica and S. aureus; with minimum inhibitory concentration (MIC) ranging from 125 to 250 μg/ml. Its polar part (86.63 %), was non-cytotoxic (50% cytotoxic concentrations: CC50 > 200 µg/mL) and displayed the strongest antibacterial activity (MIC=100μg/mL). A phytochemical investigation of the DCM extract led to the isolation of a non-toxic new diterpene named ent-kauran-15α, 16β, 17-triol (CC50 > 200 µg/mL) and a known derivative identified ent-kauran-16β, 17-diol (CC50=67.040 ± 1.257 μg / mL), which had a MIC of 100 μg / ml against S. aureus and 2 strains of Salmonella. Conclusions: The results of this study contribute to validate the traditional use of E. ferrugineum. The chemical structures of the compounds obtained may aid the design of antibacterial agents.
Keywords: Erythroxylum ferrugineum, antibacterial, Cytotoxicity, diterpenes.
BIOLOGICAL ACTIVITY OF LEAVES EXTRACTS AND ACTIVE ENT-KAURANE FROM Erythroxylum ferrugineum cav. (ERYTHROXYLACEAE)
| Lahadson Stanislas Fulgence 1* | Randrianjatovo Mamy 1 | Rabemandroso Faramalala Louise 1 | Toghueo Kouipou Rufin Marie 3 | Rakotoarison Olivier 2 | Ravelomanantsoa Germier 4 | Randrianasolo Rivoarison 1 | Nkengfack Ephrem Augustin 5 | et | Rasoanaivo Herilala Léa 1 |. Am. J. innov. res. appl. sci. 2020; 11(3):131-136.
| PDF FULL TEXT | | XML FILE | | Received | June 29, 2020 | | Accepted | August 24, 2020 | | Published | September 01, 2020 |
| Lahadson Stanislas Fulgence 1* | Randrianjatovo Mamy 1 | Rabemandroso Faramalala Louise 1 | Toghueo Kouipou Rufin Marie 3 | Rakotoarison Olivier 2 | Ravelomanantsoa Germier 4 | Randrianasolo Rivoarison 1 | Nkengfack Ephrem Augustin 5 | et | Rasoanaivo Herilala Léa 1 |. Am. J. innov. res. appl. sci. 2020; 11(3):131-136.
| PDF FULL TEXT | | XML FILE | | Received | June 29, 2020 | | Accepted | August 24, 2020 | | Published | September 01, 2020 |
*Corresponding author Author & Copyright Author © 2020: | Lahadson Stanislas Fulgence * |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied Sciences are the
property of Atlantic Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/