| ABSTRACT |Volume 1, Issue 4, Pages 158-162 (June 2015)

Research article MICRONUCLEUS FREQUENCY AND PROLIFERATION IN HUMAN LYMPHOCYTES AFTER EXPOSURE TO MIXTURES OF FIVE PERSISTENT ORGANIC POLLUTANTS (POPs) PESTICIDES IN VITRO | Najeh Hamid | Rguibi Mohamed | Naber Najat | Bakasse Mina | Harich Nourdin | and | Kandil Mostafa |. American Journal of Innovative Research and Applied Sciences. 2015; 1(4)158-162. | PDF FULL TEXT|

ABSTRACT Background: Many persistent organic pollutants (POPs) are considered as a direct cause of several non-communicable diseases particularly cancer. In this investigation we studied micronuclei (MN) as a biomarker of genotoxicity, and proliferate index (IP) as biomarker of cytotoxicity in human lymphocytes after exposure to mixtures of five POPs pesticides. Methods: The peripheral blood was collected in heparin-vacutainers from two healthy male nonsmokers volunteers aged 24 and 25 year-old. The whole blood treated with mixtures of five PoPs pesticides, Dieldrin, Heptachlor, Endrin, Aldrin, and Dichlorodiphenyl-trichloroethane (DDT), at various doses from 0.5 ‰ to 5% LD50. The cytochalasine-B micronucleus assay was used to analyze the cytogenetic alterations in lymphocytes from peripheral blood of the patients. Results: The results showed a significant dose-response in whole blood culture. The increase in micronucleated cells was accompanied by a significant inhibition of proliferate index. MN formation is multiplied three times since the concentration 0.5‰ LD50 and increased with the dose of POPs mixture. Also binuclear cells with several micronuclei are more common in cultures with the highest dose. The average cell proliferation presents a significant negative correlation with the POPs concentration, and the rate of MN presents a positive correlation. Conclusions: The results show that mixtures of five POPs exposure induce genotoxicity and cytotoxicity in human peripheral blood lymphocytes. These findings emphasize that human use of pesticides and the presence of their residues in food, water and air must be regulated and controlled. Key Words: Genotoxicity, cytotoxicity, persistent organic pollutants Mixture effect.