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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 10, Issue 6, Pages 242-249 (June 2020) |
| Research Article 6 |
|
American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
| HOME || ABOUT US || ARCHIVES || AIMS AND SCOP || AUTHORS || REVIEW|| SUBMIT MANUSCRIPT || EDITORIAL BOARD || CONTACT US |
| JUNE | VOLUME 10 | N° 6 | 2020 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference CIF/15/0289M |
American Journal of Innovative Research & Applied Sciences
Authors Contact
*Correspondant author and authors Copyright © 2020:
| Saada Nour 1* | Maowia Mukhtar 2 | Mohamed ElMakk 3 | Elshibli Mohamed 4, Walyeldin Elnour 5 | Mahdi Shamad 6 | Nuha Ibrahim 7 | Mohamed Fadl 8 | and | Safia Ahmed 9 |
Affiliation.
1. University of Bahri | College of Medicine | Department of Microbiology | Khartoum | Sudan |
2. University of Khartoum | Institute of Endemic Diseases | Department of infectious Diseases | Khartoum | Sudan |
3. University of Khartoum | Faculty of Medicine | Department of Surgary | Khartoum | Sudan |
4. Alsafa Academy Khartoum | Sudan |
5. University of Bahri | College of Medicine | Department of Pediatric | Khartoum | Sudan |
6. University of Bahri | College of Medicine | Department of Dermatology | Khartoum | Sudan |
7. National Public Health Laboratory| National Tuberculosis Reference Laboratory | Khartoum | Sudan |
8. University of Khartoum | Faculty of Medicine | Khartoum Sudan |
9. National Public Health Laboratory | Bacteriology Reference Laboratory | Khartoum | Sudan |
This article is made freely available as part of this journal's Open Access: ID | Saada-Ref.1-ajira250520 |
*Correspondant author and authors Copyright © 2020:
| Saada Nour 1* | Maowia Mukhtar 2 | Mohamed ElMakk 3 | Elshibli Mohamed 4, Walyeldin Elnour 5 | Mahdi Shamad 6 | Nuha Ibrahim 7 | Mohamed Fadl 8 | and | Safia Ahmed 9 |
Affiliation.
1. University of Bahri | College of Medicine | Department of Microbiology | Khartoum | Sudan |
2. University of Khartoum | Institute of Endemic Diseases | Department of infectious Diseases | Khartoum | Sudan |
3. University of Khartoum | Faculty of Medicine | Department of Surgary | Khartoum | Sudan |
4. Alsafa Academy Khartoum | Sudan |
5. University of Bahri | College of Medicine | Department of Pediatric | Khartoum | Sudan |
6. University of Bahri | College of Medicine | Department of Dermatology | Khartoum | Sudan |
7. National Public Health Laboratory| National Tuberculosis Reference Laboratory | Khartoum | Sudan |
8. University of Khartoum | Faculty of Medicine | Khartoum Sudan |
9. National Public Health Laboratory | Bacteriology Reference Laboratory | Khartoum | Sudan |
This article is made freely available as part of this journal's Open Access: ID | Saada-Ref.1-ajira250520 |
ABSTRACT
Background Diabetic foot ulcer infection causes great morbidity and mortality among diabetic patients and it is a major cause of lower extremity amputation worldwide. Objectives: This study aimed to determine the aerobic bacterial profile in diabetic foot infection DFI in different Wagner's grades to speculate the relation between the bacterial organisms and the depth of the wounds and to find out the correlation between the peripheral neuropathy and different ulcer grades in diabetic patients. Methods: This study was conducted in the period from December 2017 - March 2018 in a Diabetes Center in Sudan. A total of 152 diabetic patients who admitted with different grades of foot ulcers were randomly enrolled in the study. The patients were grouped using Wagner's classification. Tissue biopsies and deep swabs were collected from the ulcers for aerobic cultures. The cultured isolates were identified using phenotypic and biochemical properties. The sensation of patients was examined clinically. Results: The mean age of the patients was 54.31 (SD ± 12.1) years, males to females’ ratio was 8: 1. The mean duration of diabetes was 14 (SD ± 8) years. The ulcers varied in duration from 1 day to 10 years. Of 152 samples 181 aerobic bacteria were isolated. Cultures yielded 1-3 isolate per culture. The maximum number of bacteria was isolated from grade 3 ulcers followed by long standing ulcers 50.8% and 28% respectively. Polymicrobial infection was higher in long standing ulcers (30.4%). The isolates were mostly Gram-negative bacteria. The most frequent pathogens were proteus spp. (35.3%), Methicillin Resistant Staphylococcus aureus MRSA 14.4% and Coliform 12.2%, respectively. The most common isolates in grade 3 ulcers were P. Mirablis, Staphylococcus and Coliform, while in long standing ulcers they were P. Mirablis, S. aureus MRSA and Coagulase negative staphylococcus. The most common associated bacteria in polymicrobial infection were P. Mirablis with P. Aerginosa, S. aureos MRSA and Coliform respectively, respectively. Conclusion: Gram-negative bacteria were more prevalent and the most frequent pathogen was Proteus spp. The most common polymicrobial infections were due to P. mirablis with; P. aerginosa, S. MRSA and Coliform respectively. Major isolates in low grades 1 and 2 are Gram-positive and in high grades 3, 4 are Gram-negative bacteria. No significant relation between Wagner grades and neuropathy was detected.
Keywords: diabetic foot ulcer, aerobic bacteria, Wagner's classification.
Background Diabetic foot ulcer infection causes great morbidity and mortality among diabetic patients and it is a major cause of lower extremity amputation worldwide. Objectives: This study aimed to determine the aerobic bacterial profile in diabetic foot infection DFI in different Wagner's grades to speculate the relation between the bacterial organisms and the depth of the wounds and to find out the correlation between the peripheral neuropathy and different ulcer grades in diabetic patients. Methods: This study was conducted in the period from December 2017 - March 2018 in a Diabetes Center in Sudan. A total of 152 diabetic patients who admitted with different grades of foot ulcers were randomly enrolled in the study. The patients were grouped using Wagner's classification. Tissue biopsies and deep swabs were collected from the ulcers for aerobic cultures. The cultured isolates were identified using phenotypic and biochemical properties. The sensation of patients was examined clinically. Results: The mean age of the patients was 54.31 (SD ± 12.1) years, males to females’ ratio was 8: 1. The mean duration of diabetes was 14 (SD ± 8) years. The ulcers varied in duration from 1 day to 10 years. Of 152 samples 181 aerobic bacteria were isolated. Cultures yielded 1-3 isolate per culture. The maximum number of bacteria was isolated from grade 3 ulcers followed by long standing ulcers 50.8% and 28% respectively. Polymicrobial infection was higher in long standing ulcers (30.4%). The isolates were mostly Gram-negative bacteria. The most frequent pathogens were proteus spp. (35.3%), Methicillin Resistant Staphylococcus aureus MRSA 14.4% and Coliform 12.2%, respectively. The most common isolates in grade 3 ulcers were P. Mirablis, Staphylococcus and Coliform, while in long standing ulcers they were P. Mirablis, S. aureus MRSA and Coagulase negative staphylococcus. The most common associated bacteria in polymicrobial infection were P. Mirablis with P. Aerginosa, S. aureos MRSA and Coliform respectively, respectively. Conclusion: Gram-negative bacteria were more prevalent and the most frequent pathogen was Proteus spp. The most common polymicrobial infections were due to P. mirablis with; P. aerginosa, S. MRSA and Coliform respectively. Major isolates in low grades 1 and 2 are Gram-positive and in high grades 3, 4 are Gram-negative bacteria. No significant relation between Wagner grades and neuropathy was detected.
Keywords: diabetic foot ulcer, aerobic bacteria, Wagner's classification.
Corresponding author Author & Copyright Author © 2020: | Saada Nour * |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied Sciences are the property of Atlantic
Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/
AEROBIC BACTERIAL PROFILE IN DIFFERENT GRAGES OF DIABETIC FOOT ULCERS OF SUDANEASE PATIENTS: A CROSS SECTIONAL STUDY
| Saada Nour 1* | Maowia Mukhtar 2 | Mohamed ElMakk 3 | Elshibli Mohamed 4, Walyeldin Elnour 5 | Mahdi Shamad 6 | Nuha Ibrahim 7 | Mohamed Fadl 8 | and | Safia Ahmed 9 |. Am. J. innov. res. appl. sci. 2020; 10(6):242-249.
| PDF FULL TEXT | | XML FILE | | Received | May 25, 2020 | | Accepted | June 06, 2020 | | Published | June 13, 2020 |
| Saada Nour 1* | Maowia Mukhtar 2 | Mohamed ElMakk 3 | Elshibli Mohamed 4, Walyeldin Elnour 5 | Mahdi Shamad 6 | Nuha Ibrahim 7 | Mohamed Fadl 8 | and | Safia Ahmed 9 |. Am. J. innov. res. appl. sci. 2020; 10(6):242-249.
| PDF FULL TEXT | | XML FILE | | Received | May 25, 2020 | | Accepted | June 06, 2020 | | Published | June 13, 2020 |