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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 10, Issue 6, Pages 250-257 (June 2020) |
| Research Article |
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American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
| HOME || ABOUT US || ARCHIVES || AIMS AND SCOP || AUTHORS || REVIEW|| SUBMIT MANUSCRIPT || EDITORIAL BOARD || CONTACT US |
| JUNE | VOLUME 10 | N° 6 | 2020 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference CIF/15/0289M |
American Journal of Innovative Research & Applied Sciences
Authors Contact
*Correspondant author and authors Copyright © 2020:
| Adiouma, Dangue 1*| Mame Arama Fall, Ndiaye 1 | Ndiogou, Guèye 1| Ahmed Tidjane, Diallo 13 | and | Tahir Abdoulaye, Diop 12 |
Affiliation.
1. Cheikh Anta Diop University of Dakar | Department of Plant Biology | Mushroom Biotechnology Laboratory | Dakar BP 5005| Senegal |
2. Amadou Mahtar MBOW University of Dakar | department higher school of agricultural and food sciences | Diamniadio | Senegal |
3. University of Thiès | National Higher School of Agriculture, department of plant Production | Thiès PB A296| Senegal |
This article is made freely available as part of this journal's Open Access: ID | Adiouma-Ref.1-ajira030620 |
*Correspondant author and authors Copyright © 2020:
| Adiouma, Dangue 1*| Mame Arama Fall, Ndiaye 1 | Ndiogou, Guèye 1| Ahmed Tidjane, Diallo 13 | and | Tahir Abdoulaye, Diop 12 |
Affiliation.
1. Cheikh Anta Diop University of Dakar | Department of Plant Biology | Mushroom Biotechnology Laboratory | Dakar BP 5005| Senegal |
2. Amadou Mahtar MBOW University of Dakar | department higher school of agricultural and food sciences | Diamniadio | Senegal |
3. University of Thiès | National Higher School of Agriculture, department of plant Production | Thiès PB A296| Senegal |
This article is made freely available as part of this journal's Open Access: ID | Adiouma-Ref.1-ajira030620 |
ABSTRACT
Background: Genotoxic and cytotoxic effects of triacetin (E1518) (glycerol triacetate) food additive in Allium cepa root tip cells were researched. Objectives: The aim of this study is to investigate whether triacetin food additive has genotoxic and cytotoxic effects on Allium cepa root tip cells. Methods: Root tips were treated with different doses of triacetin (0.020, 0.025, 0.030, 0.035, 0.040, 0.048, 0.050, 0.060, 0.065, 0.75 g/l). In order to observe the effect of doses on root tip length, EC50 (effective concentration) was determined as 0.050 g/l by measuring root tips. Theroot tips were then kept at doses of EC50/2 (0.025 g / l), EC50 (0.050 g / l), 2XEC50 (0.100 g / l) for 24, 48, 72 hours. Mitotic cells were observed with a light microscope. At least 5000 cells were counted for each group. Cells in interphase, prophase, metaphase, anaphase and telophase were examined. Results: Mitotic index (MI) and chromosomal anomalyindex (CAI) were determined. Repeatedly measured ANOVA and TUKEY multiple comparison test was applied. When evaluated statistically, it was found that the difference between doses varies according to the time applied. In terms of MI and CAI, it was demonstrated that the mean of the control treatment was considerably higher in all treatment times compared to other doses treated and decreased with increasing dose concentration. As a result of treatment with EC50X2 dose, it was determined that it decreased statistically due to the increase in treatment time. Conclusion: Triacetin was found to reduce cell division in root tips compared to the control group. Thus, it has been demonstrated that triacetin has cytotoxic and genotoxic effects. It was found that cytotoxic and genotoxic effects of triacetin increased depending on the time of treatment and dose, and thus, the mitotic index decreased and the chromosomal anomaly index increased.
Keywords: Allium cepa test system, triacetin, genotoxic, cytotoxic
Background: Genotoxic and cytotoxic effects of triacetin (E1518) (glycerol triacetate) food additive in Allium cepa root tip cells were researched. Objectives: The aim of this study is to investigate whether triacetin food additive has genotoxic and cytotoxic effects on Allium cepa root tip cells. Methods: Root tips were treated with different doses of triacetin (0.020, 0.025, 0.030, 0.035, 0.040, 0.048, 0.050, 0.060, 0.065, 0.75 g/l). In order to observe the effect of doses on root tip length, EC50 (effective concentration) was determined as 0.050 g/l by measuring root tips. Theroot tips were then kept at doses of EC50/2 (0.025 g / l), EC50 (0.050 g / l), 2XEC50 (0.100 g / l) for 24, 48, 72 hours. Mitotic cells were observed with a light microscope. At least 5000 cells were counted for each group. Cells in interphase, prophase, metaphase, anaphase and telophase were examined. Results: Mitotic index (MI) and chromosomal anomalyindex (CAI) were determined. Repeatedly measured ANOVA and TUKEY multiple comparison test was applied. When evaluated statistically, it was found that the difference between doses varies according to the time applied. In terms of MI and CAI, it was demonstrated that the mean of the control treatment was considerably higher in all treatment times compared to other doses treated and decreased with increasing dose concentration. As a result of treatment with EC50X2 dose, it was determined that it decreased statistically due to the increase in treatment time. Conclusion: Triacetin was found to reduce cell division in root tips compared to the control group. Thus, it has been demonstrated that triacetin has cytotoxic and genotoxic effects. It was found that cytotoxic and genotoxic effects of triacetin increased depending on the time of treatment and dose, and thus, the mitotic index decreased and the chromosomal anomaly index increased.
Keywords: Allium cepa test system, triacetin, genotoxic, cytotoxic
Corresponding author Author & Copyright Author © 2020: | Anifowose, F. A * |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied Sciences are the property of Atlantic
Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/
EFFECT OF SODIUM CHLORIDE ON THE GROWTH AND PHYSIOLOGY OF FOUR AFRICAN VARIETIES OF Sesamum indicum L.
| Adiouma, Dangue 1*| Mame Arama Fall, Ndiaye 1 | Ndiogou, Guèye 1| Ahmed Tidjane, Diallo 13 | and | Tahir Abdoulaye, Diop 12 |. Am. J. innov. res. appl. sci. 2020; 10(6):250-257.
| PDF FULL TEXT | | XML FILE | | Received | May 01, 2020 | | Accepted | June 15, 2020 | | Published | June 18, 2020 |
| Adiouma, Dangue 1*| Mame Arama Fall, Ndiaye 1 | Ndiogou, Guèye 1| Ahmed Tidjane, Diallo 13 | and | Tahir Abdoulaye, Diop 12 |. Am. J. innov. res. appl. sci. 2020; 10(6):250-257.
| PDF FULL TEXT | | XML FILE | | Received | May 01, 2020 | | Accepted | June 15, 2020 | | Published | June 18, 2020 |