| ARTICLES | Volume 1, Issue 7, Pages 251-156 (September 2015)

Research Article TOXICITY OF THE FUNGUS BEAUVERIA BASSIANA AND THREE OILS EXTRACT AGAINST SITOPHILUS GRANARIES UNDER LABORATORY AND STORE CONDITION | Magda Sabbour | and |  |Mohamed Abdel-Raheem   |. American Journal of Innovative Research and Applied Sciences. 2015; 1(7):251-256. | PDF FULL TEXT |

ABSTRACT Background: Almost all the insect pests of stored grains have a remarkably high rate of multiplication and within one season they may destroy 10-15% of the grains and contaminate the rest with undesirable odors and flavors. Objective: The present studies aims to evaluate the toxicity of the entomopathogenic fungus, B. bassiana and three oil extract Sitophilus granarius Methods: Sitophilus granarius was reared on rice seeds at 28 ± 2°C and 60 ± 5% R.H. under laboratory conditions. Three essential oils were used in the bioassay tests: Nigella sativa, Taxodium distichum, Boswella carterii. The entomopathogenic fungus were obtained from University of Florida: B. bassiana (Deuteromycotina: Hyphomycetes) were tested against S. granarius adults. Results: Table 2 show that the effect of the grain weevil S. granarius were significantly decreased after three oil extract treatments , the percentage of larval mortality , 88.14, 39.13 and 2.21 after N. Sativa  treatments at the concentrations of 5, 0.5 and 0.05%. The corresponding concentrations of T. distichum  gave the larval mortality 57.18, 20.26 and 4.41 % of the S. granarius., respectively  (Table1)  when B. carterii treated with the last concentrations the larval mortality were significant decrease to 29.61, 15.81 and 0.613% as compared to zero in the control .The oviposition deterrent effect showed in (Table 2) the means number of eggs were significantly decreased to 13.0±7.8 , 37±7.9 and 45±3.8 eggs/ female as when treated with 3% of the oil N. sativa , T. distichum and B. carterii ., respectively compared to 198±4.9 in the control . Table 3, show the half-life period of the target insect pest after oil extract treatments which show that the LC50 obtained 21 X 104, 32 X 104, and 37 X 104  as compared to 88 X 104 sores/ml. Conclusion: the larval mortality decreased significant compared to control group when we used the entomopathogenic fungus and the oil extract. Key words: Beauveria bassiana; plant oils, Sitophilus granarius.