This article is made freely available as part of this journal's Open Access: ID | Mustapha ManuscriptRef.1ajiras140616 |
1,2 University Sultan Zainal Abidin l Scholl of Animal Science l Kuala Terengganu, 21300 l Malaysia l
3. Department of Animal Husbandry l Sam Hingginbottom Institute of Agriculture l Technology and Science- Deemed-University l Allahabad-21107(U.P) l
4. Department of Medical l Laboratory Science l College of Medicine l Bayero University Kano l India l
Authors Copyright © 2015:
| Mustapha Jamilu Adamu | Zarizal Suhaili | Abdulhamid Sule Umar | and | Rufa’I Umar Zubair |
American Journal of Innovative Research & Applied Sciences
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American Journal of innovative
Research & Applied Sciences
ISSN 2429-5396 (Online)
| June | VOLUME 1 | N° 6| 2016 |
Background: Genetic variation within and among population is important for conservation, genetic improvement and species adaptability. The effectiveness of selection and crossbreeding depend on the amount of genetic variation.In the current study, genetic diversity of indigenous chicken (Gallus gallus gallus) from East Coast Peninsular Malaysia (ECPM)was estimated using mitochondrial DNA (mtDNA) analysis. Method: First 546 bp of the control region of mtDNA were analyzed in 59 chickens collected from three populations of Kelantan, Pahang and Terengganu. Result: Eleven haplotypes were detected from all samples. Hap-1 is the most common among the three populations and comprised 45.8% of the total samples used. The average haplotype and nucleotide diversity were0.7645 and 0.00836 respectively. Non-significantTajima’s D and Fu’s FSvalueswere observed in Pahang and Terengganu populations. The significant Tajima’s D value observed in Kelantan indicatedsignificant (p<0.05) departure from neutrality model. The haplotype neighbour joining (NJ) tree revealed shallow topology, and no significant genealogical clusters of samples corresponding to sampling locations was observed. Conclusion: Higher genetic diversity was observed in this study. Low gene diversity observed in Kelantan might indicate the evidence of population bottleneck due to flood disaster in the area. Additionally, based on the NJ tree, indigenous chicken from ECPM are closer to the Gallus gallus gallus and relatively far away from Gallus gallus bankiva.
Key words: indigenous chicken, mitochondrial DNA, control region, genetic diversity.