| ABSTRACT | Volume 1, Issue 3, Pages 75-79 (May 2015)

Research Article Development and Validation of Stability Indicating Spectrophotometric Method for Pyridoxine Hydrochloride in Bulk and Pharmaceutical Formulations | Shabana Naz Shah |. The American Journal of Innovative Research and Applied Sciences. 2015; 1(3):75-79. | PDF FULL TEXT |

ABSTRACT Background: Pyridoxine Hydrochloride is a water soluble vitamin which is also known as Vitamin B6. Objectives: In this recent research developed a very simple, least time consuming, inexpensive   and efficient UV Spectrophotometric method for the assay of pyridoxine in bulk form and in its pharmaceutical dosage formulation. Methods: The assay is based on the Ultraviolet Spectroscopy (UV),   measuring lmax at 290 nm for pyridoxine. A sample of bulk was dissolved in 0.1 N HCl to produce a desired concentration of solution containing pyridoxine. Similarly, a sample of pharmaceutical   formulation was grind and dissolved in 0.1 N HCl and various dilutions were made. Results: The absorbance of bulk, dosage formulation preparation was measured at 290nm against 0.1 N HCL (blank).   Calibration curves were linear over the range of 2-14 μgmL-1 with a correlation coefficient is ≥ 0.9997 at lmax 290 nm. Conclusion: The analysis shows a linear relationship between the absorbance   and concentration. The proposed method was statistically validated and successfully applied for routine analysis of pyridoxine in bulk and tablet dosage forms. Keywords: Pyridoxine, Bulk, Dosage Formulation, UV spectrophotometer.

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